The Hybrid bioreactor is a versatile bioreactor that can do four types of culture, namely: adherent culture using macrocarriers, adherent culture using microcarriers, suspension culture, and fermentation. This system is best used for research and development before scaling up to a larger bioreactor system.
Technical Specifications
|
|
| Control Unit Weight | |
| Single | 80 kg (177lbs) |
| Control Unit Dimensions | |
| Single | W460x D 637 x H 903 mm |
| Utility Connections | |
| Power Supply | 220 volts, max power consumption 5A |
| Gases |
4x6mm external tubing diameter Gas supply pressure, 10psi oil & moisture-free |
| Water |
Water supply pressure- 1.5 M Flow rate up to 10-12 lpm Minimum temperature 13-14°C L/S 35 Silicone for tubing |
| Control Unit | |
| Housing | Stainless Steel, AISI 304 |
| Display | HMI |
| Resolution | 800x600 |
| SCADA Communication | Ethernet |
| Safety valve gas pressure | 10 psi for Matrix Reactor Vessel and Suspension Vessel |
| Water inlet pressure reduction | 0.15bar, integrated pressure control |
| Motor Drive | |
| Direct Drive | Power: 400W |
Technical Specifications |
|
| Reactor Vessel Material | |
|
1 Liter and 7 Liter vessel Total Volume: 1.5L and 8.2L |
Borosilicate glass |
| 14 Liter vessel | Borosilicate glass/Stainless Steel |
| 25 Liter vessel | Stainless Steel |
| 50 Liter vessel | Stainless Steel |
| Vessel Dimensions | |
| 1 Liter | D 116 x H 148 mm |
| 7 Liter | D 190 x H 320 mm |
| 14 Liter | D 240 x H 450mm |
|
Sensor | Measurement Range | Display Accuracy |
Description | |
|---|---|---|
| Temperature | PT-100 class A ; Deviation: ±(0.15+0.00 |t| ); 0°C-100°C | |
| Dissolved oxygen | Measurement Principle | Amperometric/ Polarographic |
| Working Conditions | ||
| Pressure resistance measurement | 0.2-6 bar absolute | |
| (2.9-87.0 psi absolute) | ||
| Mechanical resistance measurement | max. 12 bar absolute | |
| (174.0 psi absolute) | ||
| Measuring temperature range | 0...80°C (32…176 °F) | |
| Temperature range |
-5…140°C (23…284°F) (autoclavable) |
|
| Construction | ||
| Temperature compensation | Automatic with built-in RTD | |
| Cable connection |
VarioPin (IP 68) straight or angled K8S straight (digital ISM sensors) |
|
| O-ring materia | Silicone FDA and USPP VI approved | |
| Membrane material |
PTFE/Silicone/PTFE (reinforced with steel mesh) |
|
| Wetted metal parts |
Stainless steel Special material on request |
|
| Surface roughness of wetted metal parts (ISO 1320:1992) | N5 (< 0.4m [16in]) | |
| Quick disconnect interior body | Standard | |
| Cathode | Pt | |
| Anode | Ag | |
| Guard ring | No | |
| Dimensions | ||
| Sensor diameter | 12 or 25 mm (0.47” or 0.96”) | |
| Immersion length (a) for 12 mm sensor |
70, 120, 220, 320, 420 mm. (2.8, 4.7, 8.66, 12.6, 16.54”) |
|
| Immersion length (a) for 25 mm sensor |
80, 160, 260, 360 mm (3.15, 6.3, 10.24, 10.17”) |
|
| Performances | ||
| Detection limit | 6ppb | |
| Accuracy |
≤ ±(1% + 6 ppb) of reading in liquids |
|
| Response time at 25°C/77°F (air – N2) | 98% of final value <90s | |
| Sensor signal in ambient air (25°C/ 77°F) | 50…110nA | |
| Residual signal in oxygen-free medium | <0.1% of the signal in ambient air | |
| Maximum flow error | ≤ 5% | |
| Certification | ||
| EHEDG, 3A | Yes | |
| 3.1B (EN 10204.3/1.b) | Yes | |
| ATEX certificate | Yes | |
| FM Approval | Yes | |
| FDA/USP VI | Yes | |
| Quality control | Yes | |
| pH | Measuring range | pH 0…14 |
| Temperature |
0…80°C for operation 0…140°C for sterilization |
|
| Pressure resistance | Up to overpressure of max. 6 bar (87psi) | |
| Reference system | Argenthal (Ag/AgCl) | |
| Reference electrolyte | Gel electrolyte | |
| Diaphragm | 1 ceramic diaphragm | |
| Glass membrane | HA, high alkali glass quality | |
| Glass shaft | Ø12 mm | |
| Plug head | K8S, Pg 13.5 thread | |
| Foam Control | Teflon insulated stainless steel | |
| Level | Teflon insulated stainless steel | |
| Turbidity | Dimension | W 270 x D 200 x H 300 mm |
| Measuring principle | Backscatter, 1-fiber | |
| Sensor material | DIN 1.4435 (316L) | |
| Sensor diameter | 12 mm | |
| Insertion length | 120, 205, 297, or 407 mm | |
| Process connection | Pg 13.5 | |
| Measuring range | ||
| Formazin Turbidity Units 1 | 10…40000 FTU | |
| Suspended Solids (Diatomaceous earth as reference) | 0…250 g/L | |
| Design | ||
| Cable connectors | SMA | |
| Cable lengths | 3m (10ft) optica | |
| Cable type | Duplex HCS fiber, fixed | |
| Wetted parts | ||
| Metals | DIN 1.4435 (316L) | |
| Plastic | None | |
| O-ring | Viton-FDA | |
| Sapphire spigot for fiber | None | |
| Sapphire window for fiber protection | None | |
| Working conditions: | ||
| Pressure range | 0…6 bar (0…87psi) | |
| Redox | Dimension | W 270 x D 170 x H 230 mm |
| Measuring range | pH 0…12 | |
| Temperature |
0…100°C in operation 0…130°C for sterilization |
|
| Pressure resistance |
From vacuum up to a process pressure of max 2.5 bar (36psi) |
|
| Reference system | Argenthal (Ag/AgCl) | |
| Reference electrolyte | Pressurized gel electrolyte | |
| Diaphragm | 1 ceramic diaphragm | |
| Glass shaft | Ø12 mm | |
| Plug head | K8S, Pg 13.5 thread | |
| RM load cells |
Weight:100 kg Dimensions: (in mm) 330 X 330 |
|
| Vessel Sizing and Volume | ||
|---|---|---|
| Model | M-1000 | M-5000 |
| Total Volume (L) | 1.5 | 8.2 |
| Maximum Working Volume (L) for Suspension Culture | 0.6-1.1 | 4.5-7 |
| Depth (mm) | 116 | 190 |
| Height (mm) | 148 | 320 |
| Recommended BioNOC II Matrix volume [ml] | 180 | 1000 |
| Recommended amount BioNOC II Matrix in grams | 10 | 55 |
| Aspect Ratio | 1:2.5 (for suspension) 1:3 (for microbial culture) | |
| Specifications* of VXL Hybrid Bioreactor | Adherent Cell Culture | Suspension Cell Culture | Fermentation | Microcarrier-Based Cell Culture | ||||
|---|---|---|---|---|---|---|---|---|
| Model | M-1000 | M-5000 | M-1000 | M-5000 | M-1000 | M-5000 | M-1000 | M-5000 |
| Vessel Material | Borosilicate Glass | |||||||
| Temperature Range | 20ºC - 70ºC Precision: 0.1 ºC | 10 ºC-70 ºC Precision: 0.1 ºC | 20 ºC-70 ºC Precision: 0.1 ºC | 10 ºC-70 ºC Precision: 0.1 ºC | 20 ºC-70 ºC Precision: 0.1 ºC | 10 ºC-70 ºC Precision: 0.1 ºC | 20 ºC-70 ºC Precision: 0.1 ºC | 10 ºC-70 ºC Precision: 0.1 ºC |
| Baffle | N/A | YES | YES | N/A | ||||
| Top Stirrer Motor | 10-1200rpm | |||||||
| Impeller speed | 25-200r pm | 50-1200 rpm | 50-1200 rpm | 25-400 rpm | ||||
| Impeller Design** | Small 6-Blade Rushton Turbine | 3-Pitched-Blade Turbine and 3-Marine-Blade Propeller | Large 6-Blade Rushton Turbine | 3-Pitched-Blade Turbine and 3-Marine-Blade Propeller | ||||
| Ventillation | Ring Sparger | Micro-Sparger | Ring Sparger | Ring Sparger | ||||
| Drive Method | Tide: KNF brushless motor with air pump, controlled by PID or constant voltage direct drive. Mixing: Top direct servo motor with PID/PWM control | Mixing: Top direct servo motor with PID/PWM control | ||||||
| Condenser | Stainless steel condenser with 0.2 um disk type or cartridge type filter | |||||||
| pH Range | pH 4-14 Precision: 0.01 | |||||||
| DO Range | 0-100 % Precision: 0.01% | |||||||
| Aeration | 10L/min Precision: 2% | |||||||
| Emerging Time | 0-100mins | N/A | ||||||
| Submerging Time | 0-100mins | N/A | ||||||
| Liquid Level Control | High Level Sensor | Electronic Scale | ||||||
| Sampling | liquid: sampling bottle; matrix: sample port | liquid: sampling bottle | ||||||
| Foaming Control | N/A | YES | YES | YES | ||||
| Control Unit | 8.4” HMI | |||||||
