Adherent cell culture depends on surfaces on which cells attach and grow. These micro/macrocarriers increase the surface area for large scale cell culture, thereby reducing footprint compared to conventional 2D culture systems. Esco VacciXcell offers macrocarriers that allows growth of cells in 3D, thereby increasing target production.
BioNOC™ II is a macroporous carrier that supports the growth of anchorage-dependent cells including animal, mammalian, and insect cells in either serum-containing or serum-free culture media. It is made of 100% pure polyester nonwoven fabric manufactured according to cGMP guidelines. The special geometric design and surface treatment on the carrier enhance fluid mixing, immobilization efficiency, protection from shear forces, and nutrient transfer during cell culture.
BioNOC™ II carriers are straight and uniform fibers that favor cell distribution and growth
Microscopic view: cell attached to BioNOC™ II
Illustration of how cells are situated within BioNOC™ II
Productivity: Up to 5X108 cells/g of BioNOC™ II (for Vero cells)
Growth Area: Up to 2,400 cm2 /g of BioNOC™ II
High surface area to volume ratio: 160 cm2/ml
100% Polyethylene Terephthalate (PET) material
0.3 mm thickness for easy exposure to culture medium oxygen transfer
Special surface treatment to enhance biocompatibility
Special geometric design to enhance mixing and support load-bearing capacity in packed-bed bioreactors
Compressible for higher cell density
Hydrophilicity stable for four years
Sterilizable by autoclave, gamma irradiation, and EO sterilization
Low lint, convenient for adherent cell growth for protein or viral vaccine production
Growth of anchorage-dependent cells including animal, mammalian, and insect cells in packed-bed cell culture systems. Compatible with CelXrocker™, CelCradle™. TideXcell™. and other commercially available packed-bed culture equipment.
BioNOC™ II supports the growth of different cell lines, including CHO, CHO-K1, rCHO-hlgG, rC-127-TPA, HEK293, Hybridoma, Vero, Sf-9, Hi-5, BHK-21, rBHK Factor VIII, HepG2, Hela, Huh 7, RK-13, human foreskin fibroblasts, human muscle skeleton cells, human mesenchymal cells, and human embryonic stem cells.