Throughout a cell culture experiment, cells must be regularly analyzed both quantitatively and qualitatively. Aside from cells, the cellular environment must also be regularly monitored and kept at a constant level in order to ensure that the cells are under optimum growth conditions. Bioreactors, especially packed-bed, are complex systems due to their physical operation while carrying out biological processes internally. Thus, it is important to have effective process monitoring (Process analytical technology (PAT) and In process quality control (IPQC) and control to ensure that cell cultivation remain under controlled conditions. Esco VacciXcell offers complementary product and bioreactors with built-in features for cell monitoring.
Crystal Violet Dye Nuclei Count Kit is a simple tool for the quantification of cells based on the number of nuclei dyed. The CVD kit contains reagents that disrupt the cells, thereby releasing the nuclei, which are subsequently dyed.