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Technology Advantage

No Oxygen Limitation

Dual oxygenation action provides cells with ample oxygen regardless of the bioreactor scale.

Zero Shear Stress

Gentle upward and download tide motion (2mm/s) provides nutrients and oxygen to cells without applying shear stress, perfect for shear sensitive cells. Minimizes impurities with host cell protein and DNA/RNA.

No Bubbling, No Foaming

Tide Motion bioreactors use separate vessels for culturing and stirring this isolating the cells being cultured from any form of bubbling or foaming.

Controlled cell growth (Batch mode)

Controlled cell growth during cell culture to promote productivity in batch mode via extending exposure time during tide motion without requiring additional feed control

Immortal cell lines

Freezing cells in Go and G1 phase by nutrient starvation by deprivation of glucose to boost productivity of protein expression.

100% Continuous Processing > 3 months

Ideal for biosomilars/biobetters, monoclonal antibodies (MABs), and recombinant proteins. As cells are immobilized, cell retention devices which are typical for perfusion-based suspension cells such as Chinese Hamster Ovary (CHO) are not required to separate cells from media. This means no fouling, foaming, and scalability issues resulting from flow rate limitations of cell retention devices.

Shortest bioprocess scale up development time

parameters developed in academic / industrial R&D can be easily translated into clinical trials and commercial production as parameters are identical and hence linearly scalable without complex scale up bioprocess calculations (as those found in Stirred tanks).

Large Surface Area

5.5 g of carrier (amount of macroporous carriers in 1 CelCradle bottle) is already equivalent to up to 15,000cm2 of surface area. These carriers mimic the cells in vivo state resulting in higher cell or product yield.

Why Join the Tide?

VacciXcell Hybrid Bioreactor was Conceptualized and designed in Singapore in 2016
  • Four-in-One - VacciXcell Hybrid Bioreactor can perform four (4) types of culture processes namely adherent culture using microcarrier, adherent culture using macrocarrier, suspension culture and fermentation. Adherent culture is performed through patented Tide Motion technology while suspension culture and fermentation are performed through standard stirred tank process.
  • Perfect for Vaccine, R&D, Pre-Screening - when deciding between suspension or adherent cell lines or converting adherent cell lines to suspension, data can be easily compared. Comparison can be made by using conventional microcarriers or our packed bed BioNOC™ II (when using Tide Motion).
  • Perfect for Cell & Gene Therapy - Adeno-associated Virus (AAV) / Lentivirus (LV) or other Simian Immunodeficiency Virus (SIV) production, HEK-293T with transient transfection, autologous or allogeneic mesenchymal or exosomes production ( including anaerobic hypoxic mode).
  • Perfect for Virus Production using Adherent Cell Lines
  • Process Development for Tide Motion - Linearly scalable across different packed bed volumes
  • Perfect for New Bioprocessing Laboratories - Since the system can perform four (4) types of culture, researchers can easily compare how one cell line performs or reacts to one type of culture method as compared to another. Costs are saved by not having to purchase multiple separate bioreactors. This allows researchers to determine which type of culture is the best one to go with when scaling up. Different culture vessels for fermentation 1:3 and autoclave between batches ensures minimal cross contamination. A wide variety of culture vessels which are easily inter-changeable between Tide Mode and Suspension Mode via a top mounting plate allows ease of usage without having to purchase a variety of culture vessels. Smaller vessels for suspension culture at 0.5 L smallest can be provided
  • Temperature Control by Water Jacket - allows culturing in low or high temperatures for special cases
    • Low Temperature - for viruses which would have better yields in low temperature such as BEFV or applications which require low temperature such as in aquaculture vaccines
    • High Temperature – For applications which would require high temperatures such as in developing DNA vaccines.
  • Easy Scale Up - The adherent process of the hybrid bioreactor can easily scale up to TideCell™ bioreactors while the hybrid bioreactor is also equipped with mass flow controller for suspension & fermentation process can scale up to larger stirred tanks. ATF systems for perfusion for higher concentration of cells for less complex seed trains running in suspension.
  • Isolator Capability - VacciXcell™ Hybrid can be easily integrated inside an Esco Pharma isolators in case the application requires BSL3/4 environment or for Autologous / Allogeneic cell & gene therapy controls can be fully integrated into 1 common HMI with Esco BioVap (Hydrogen peroxide biodecon or Ammonia biodecontamination system for 6 log reduction in bioburden and/or Esco Autoclave mounted on the side).
  • International Support - Esco VacciXcell Hybrid bioreactor is sold & serviced globally by Esco’s 25 direct sales/service offices, factories and trained sales & service partners.
  • Ease of Usage - CelCradle™ was designed to be used with basic scientific knowledge, good aseptic technique, good laboratory practice, like in normal static 2D culture (T Flasks, roller bottle systems, petri dishes, stacked planar culture) that provides higher cell density. It comes with simple caps like T-flask and easy parameter controls. For example, pH is controlled manually through adjusting the CO2 concentration inside the CO2 incubator. A wide variety of CelCradle bottles allows production of various adherent cell lines, viruses, and proteins.
  • LIMS - A simplified laboratory information and management systems (LIMS) allows ease of recording of data for DOE and eventual scaling up into TideXcell™.
  • Fully Single-use Perfusion Option with Closed Cell Harvesting
  • Portable and Intuitive - CelCradle™ can easily fit inside a 6ft3 CO2 incubator for batch culture and 8ft3 CO2 incubator for recirculation culture. It has only two (2) parameters which can easily be optimized. The magnetic controller of the CelCradle™ can be magnetized to the outer wall of the CO2 incubator, allowing parameter change without opening the incubator.
  • Technology Transfer Made Easy - CelCradle™ uses the same principle as roller bottles, easily adapting whatever protocol or parameters are already established. Transferring from static culture like T-flasks or hyperstacks to CelCradle™ is also just as easy. 
  • Flexible - Capable of doing batch, fed-batch and recirculation mode by simply changing accessories.
  • High Yield in a Small Footprint - One (1) CelCradle™ bottle containing only 5.5g of macroporous carriers has a yield equivalent to twenty (20) 850cm2 roller bottles or one-hundred (100) pieces of 150cm2 T-flasks
  • 3D in vivo like Culture Environment - allows cell-to-cell contact and a large surface area to footprint ratio for culturing cells.
  • True Linear Scalability - CelCradle™ is a 0.1L packed bed bioreactor that can easily be used for seed preparation for TideXcell™ bioreactor that scale all the way up to 5,000L packed bed volume production scale. This eliminates lengthy developmental processes and parameter optimization that are common in scaling up in microcarriers and/or suspension culture. Unlike other packed bed or hollow fiber systems, seed cells are not prepared in 2D and expanded in 3D, this is 3D to 3D
  • Isolator Capability - CelCradle™ can be easily integrated inside an Esco Pharma isolator in case the application requires BSL3/4 environment or for autologous / allogeneic cell & gene therapy. Controls can be fully integrated into 1 common HMI with Esco BioVap hydrogen peroxide or biodecontamination system for 6 log reduction in bioburden and /or Esco Autoclave mounted on the side, Esco Centrifuges, Co2 Incubators, Peltier Blocks.
  • International support - Esco CelCradle™ is sold & serviced globally by Esco’s 25 direct sales/service offices, factories and trained sales & service partners.
  • 3D in vivo like Culture Environment - allows cell-to-cell contact and a large surface area to footprint ratio for culturing cells.
  • True Linear Scalability - Same parameters are used from 0.1L packed bed volume lab scale for seed preparation, all the way up to 5,000L packed bed volume production scale. This eliminates lengthy developmental processes and parameter optimization common in scaling up in microcarriers and/or suspension culture. Unlike other packed bed or hollow fiber systems, seed cells are not prepared in 2D and expanded in 3D, this is 3D to 3D.
  • Closed Cell Harvesting System - TideXcell™ Cell Harvesting System (TXCHS) is a powerful, closed, automated system for harvesting viable cells from 2L to 20L TideXcell™ bioreactors with more than 90% recovery.
  • 100% nutrient exchange for highest virus titer - Media used for cell growth can be 100% pumped out as waste and 100 % new media is used after virus seeding for virus growth. This has higher yields for VLPs compared to stirred tank which new media can only be perfused in.
  • Hybrid System - Can be configured for either single use or combination of single use & autoclavable vessels.
  • Difficult Viruses - Parameters such as temperature, pH, and oxygen levels are automatically monitored and adjusted to cater specific virus requirements. TideXcell™ also has extremely low shear stress for stress sensitive viruses and is capable of harvesting using a closed automated harvesting system for non-secreted viruses.
  • Uniform seeding - Unlike other packed bed systems, single-use TideXcell™ matrix cassettes are designed and configured to allow even seeding within the packed bed.
  • Isolator Capability - CelCradle/ TideXcell™ can be easily integrated inside an Esco Pharma isolator in case the application requires BSL3/4 environment or for autologous / allogeneic cell & gene therapy. Controls can be fully integrated into 1 common HMI with Esco BioVap™ hydrogen peroxide biodecontamination or ammonia system for 6 log reduction in bioburden and/or Esco Autoclave mounted on the side.
  • cGMP References - TideXcell™ is used in current good manufacturing practice (cGMP) Facilities for both commercial human and animal vaccines that are certified to FDA, eucGMP, Japanese Industrial Standard (JIS), Pharmaceutical Inspection Co-operation Scheme (PIC/s) standards.
  • PAT/IPQC - Process Analytical Techniques (PAT) and In Process Quality Control (IPQC) via patented single use sampling method of BioNOC™ II allows IPQC for cell counting during production unlike other packed bed bioreactors which need to be dis-assembled during process development.
  • In Situ Configurable - batch, fed-batch, perfusion, continuous processing and parameters for low temperature, anaerobic/hypoxic conditions, single use/autoclavable as well as mixing, feed & harvest tanks with auto switch pumps can be easily configured in situ via Wonderware. These are easily programmed with minimal training without coding background.
  • SCADA enabled with 21 CFR part 11 compliance and CSV - Esco Healthcare factories globally in Singapore/USA/UK provide Supervisory Control and Data Acquisition (SCADA) or Distributed Control System (DCS) Configuration and deployment with computer system validation (CSV). We are well versed in DeltaV, Pcs7 by default SCADA system deployed on VacciXcell adherent single use platform is using Wonderware running on Siemens programmable logic controller (PLC). Siemens human machine interface (HMI) can be provided for standalone non-SCADA or non-multi-functional dedicated bioprocessing applications.
  • International support - Esco TideXcell™ is sold & serviced globally by Esco’s 25 direct sales/service offices, factories and trained sales & service partners

TideXcell™
3,500 up to 5,000 L

Tide 2mm/s: 3,000 - 5,000 L (st. st. tanks)
Tide 6mm/s: 55,000 L

Large st. st. tank,scale for secreted virus production, viral vectors, cellular agriculture (optional edible scaffolds), or biosimilars/biobetters

TideXcell™
300 L

TideXcell™
100 L

TideXcell™
20 L

TideXcell™
2 L

CelCradle ™

with Semi-automated Harvester Esco standalone bioreactor. Single Bottle, Optional Docking to Isolator.

CelCradle™ + CelShaker™

Early stage research and development. One stage can occupy 4 CelCradle bottles, integrated inside client's CO2 incubator. Manual control

CelXrocker™

2D Tide proof of concept (POC) quick data seed ~20,000-60,000 cells per carrier