Dual oxygenation action provides cells with ample oxygen regardless of the bioreactor scale.
Gentle upward and download tide motion (2mm/s) provides nutrients and oxygen to cells without applying shear stress, perfect for shear sensitive cells. Minimizes impurities with host cell protein and DNA/RNA.
Tide Motion bioreactors use separate vessels for culturing and stirring this isolating the cells being cultured from any form of bubbling or foaming.
Controlled cell growth during cell culture to promote productivity in batch mode via extending exposure time during tide motion without requiring additional feed control
Freezing cells in Go and G1 phase by nutrient starvation by deprivation of glucose to boost productivity of protein expression.
Ideal for biosomilars/biobetters, monoclonal antibodies (MABs), and recombinant proteins. As cells are immobilized, cell retention devices which are typical for perfusion-based suspension cells such as Chinese Hamster Ovary (CHO) are not required to separate cells from media. This means no fouling, foaming, and scalability issues resulting from flow rate limitations of cell retention devices.
parameters developed in academic / industrial R&D can be easily translated into clinical trials and commercial production as parameters are identical and hence linearly scalable without complex scale up bioprocess calculations (as those found in Stirred tanks).
5.5 g of carrier (amount of macroporous carriers in 1 CelCradle bottle) is already equivalent to up to 15,000cm2 of surface area. These carriers mimic the cells in vivo state resulting in higher cell or product yield.
3,500 up to 5,000 L
Tide 2mm/s: 3,000 - 5,000 L (st. st. tanks)
Tide 6mm/s: 55,000 L
Large st. st. tank,scale for secreted virus production, viral vectors, cellular agriculture (optional edible scaffolds), or biosimilars/biobetters
with Semi-automated Harvester Esco standalone bioreactor. Single Bottle, Optional Docking to Isolator.
CelCradle™ + CelShaker™
Early stage research and development. One stage can occupy 4 CelCradle bottles, integrated inside client's CO2 incubator. Manual control
2D Tide proof of concept (POC) quick data seed ~20,000-60,000 cells per carrier