Throughout a cell culture experiment, cells must be regularly analyzed both quantitatively and qualitatively. Aside from cells, the cellular environment must also be regularly monitored and kept at a constant level in order to ensure that the cells are under optimum growth conditions. Bioreactors, especially packed-bed, are complex systems due to their physical operation while carrying out biological processes internally. Thus, it is important to have effective process monitoring (Process analytical technology (PAT) and In process quality control (IPQC) and control to ensure that cell cultivation remain under controlled conditions. Esco VacciXcell offers complementary products and bioreactors with built-in features for cell monitoring.
Tide Motion systems, through the use of BioNOC™ II macrocarriers, allow individual carrier sampling for PAT/IPQC purposes. Cell counting can be done by sampling individual BioNOC™ II and staining it with crystal violet dye (CVD).
Note: Stain to be used for cell monitoring will depend on the cell line used.