Egg based production has been the traditional form of virus production specially for influenza vaccines. 12 day old fertilized pathogen free eggs are marked, pierced and inoculated with the desired virus. The virus is then allowed to replicate for about 48 hours at 37 degrees. The allantoic fluid that contains the virus is then harvested. Two eggs worth of viruses is approximately equal to 1 dose of vaccine.
Esco VacciXcell’s Tide Motion bioreactor is the perfect companion for all sorts of virus production for vaccines. Due to the proprietary macroporous carrier technology, BioNOC™ II, a 0.1 L bioreactor can culture up to 5x109 cell density. Being a linearly scalable bioreactor, development process and parameter optimization is reduced to a minimum during scaling up, reducing both process time and development cost.