It requires a stable cell line such as Vero, MDCK (adherent lines which are widely used) or PER.C6 (suspension line) capable of propagating the virus and can scale up to manufacturing scale instead of replicating the amount of systems (scale-out) needed for vaccine production
The most common CCLs (continuous cell lines) used are VERO and MDCK cells lines which can both be cultured in cGMP Tide Motion bioreactors. The bioreactor utilizes the Tide motion principle that provides gentle oscillation of the medium, with an extremely low shear stress, foaming, and bubble-free environment for the cells. Both VERO and MDCK cells have been optimized in a culture system using CelCradle™ bioreactor that houses BioNOC™ II macrocarriers for cell growth which mimics 3D in vivo environment.
The expansion of adherent cells such as VERO and MDCK in the CelCradle™ can be achieved through linear adherent scale-up using the TideXcell™ bioreactor. The system is equipped with 100% media exchange for high viral titer. This demonstrates that innovative technologies being developed today can improve the efficiency of historically established egg-based systems.