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3D Cell Culture Systems | Esco VacciXcell

3D Cell Culture Systems

The reason for the growth in 3D cell culture is its better imitation of the parental tissue architecture. 2D cell cultures only exist in two dimensions and does not feature proper cell-cell and cell-environment interactions. 3D models provide a better representation of how cells grow or how they are affected by disease and injury.

Although 2D cell cultures are still used for most research, the 3D cell culture industry is starting to catch up, especially in cancer treatment and stem cell research. Reasons for the increasing acceptance and use of 3D cell cultures include:

More relevant cell models
- 3D models offer a simpler, lower cost biological research for disease models

Cell characteristics
- 2D systems have changes in terms of morphology and phenotype diversity. 3D systems provide preserved cell morphology and diverse phenotype and polarity

Cells interaction
- 3D systems offer a better model and provide proper interactions of cell-cell and cell-extracellular environment. Creation of complex systems linked together by microfluidics means that 3D tissue systems can provide environmental niches

Access to essential compounds
- Precise control and manipulation of fluid to govern mass transport is better in 3D. Same as in vivo, there is variable access to nutrients, oxygen, and metabolites to where they’re needed, meaning cells and organs grow in a more realistic way

In vivo imitation
- Representation of tissues and organs are greatly enhanced in 3D culture. Expression of genes, splicing, topology, and biochemistry is an advantageous molecular mechanism of 3D culture systems. Proper functioning plays a vital role in understanding many diseases

Cell-based models instead of animal models
- Due to the advantages mentioned above, cell-based models provide a reliable way to predict how a drug will work and affect humans/animals

There are some disadvantages for 3D cell culture systems, but Esco VacciXcell provides solutions for these:

Culture formation and throughput
- Available 3D culture systems are time-consuming however, Esco VacciXcell Tide Motion bioreactors provide high throughput needed for large-scale bioprocessing especially for culturing cells. The CelCradle™ system can occupy 4 bottles for high product yield and scale up to the TideXcell™ system

Cell monitoring and observation
- BioNOC™ II macroporous carriers allow microscopic observation of cells and monitoring of growth and development. It has been created to provide ease of analysis and better readouts across all cells being cultured in the system

Access to essential compounds
- When phasing to manufacturing scale, it can be a challenge to determine if there is homogeneity in nutrient and oxygen distribution. The Tide Motion principle is akin to an “artificial lung” thereby giving off dual oxygenation in the system and alternatively exposing the cells to nutrition

Scaffold material
- BioNOC™ II macroporous carriers is made of 100% PET, animal-, human-component free rendering it safe for cell culture usage. The manner the cells grow are not restricted and doesn’t affect cell adhesion, growth, and behavior. The carriers are also hydrophilic and biocompatible. Coating factors can be used when necessary