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TideXcell™

The Gentle Giant of Adherent Bioprocessing

TideXcell™ is a linearly scalable system that runs on Tide Motion principle. It is ideal for biomass expansion of adherent cells under single-use or multiple-use technologies.

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Tide Motion in TideXcell™

Features

  • Can be single-use or multiple-use
  • Adherent cell scale-up for up to 5,000L packed-bed volume (bio-equivalency of 50,000 L in suspension)
  • a 100% media exchange system ideal for continuous bioprocessing
  • Separation of the matrix and mixing vessel permits dual temperature control process to produce higher virus titers
  • Built-in weighing platform and inhouse mixer
  • PLC based monitoring system with somple, intuitive touchscreen runs on Wonderware SCADA
  • Can be connected to SCADA systems with DeltaV or Pcs7 controls
  • Dual redundant systems for critical components such as PLC and pumps are catered
  • Provides an ultra-scale down process and reduces costs by saving on media, labor, space, utilities, and most importantly, eliminates cross-contamination
  • Isolator capability
  • cGMP references
  • PAT/IPQC

Applications

  • Culture of anchorage-dependent cells with BioNOC™ || carriers
  • Cell mass train
  • Vaccine production
  • Recombinant protein and monoclinal antibody production
  • Biosimilars and biobetters
  • Transition from 2D system to closed 3D system
  • Continuous bioprocessing
  • Wild virus production
  • Cell therapy
  • Biologics for neglected tropical diseases and orphan diseases

Highlights

Uniform Seeding

TideXcell™ Matrix Bed is designed to cut the area into numerous small sections and force cells to flow in horizontal radial direction instead of vertical direction. The gradient effect during seeding phase could be minimized.

A matrix cassette is packed with carriers configured in 3D for optimum cell growth. Cassettes are stacked inside the matrix vessel(2 L, 20 L, 100L) for uniform seeding of cells.

Uniform Seeding Principle

TideXcell™ Matrix Bed is designed to cut the area into numerous small sections and force cells to flow in horizontal radial direction instead of vertical direction. The gradient effect during seeding phase could be minimized.

TideXcell™-100 Packed with Matrix Cassettes
(Top View)

The concept is achieve by packing the matrix bed with porous cassettes with smaller diameter when matrixes are packed inside.

100% Media Exchange

Continuous harvesting can be done in the TideXcell system due to 100% media exchange. This process is beneficial when doing viral culture work for producing higher viral titers or for stem cell culture which requires switching to a different media for differentiation.

Due to the seperation of the matrix vessel (where cells reside) and the mixing vessel (where the mixing of the culture medium is happening), the system is capable of fully harvesting the waste or conditioned media and feeding in a 100% fresh culture medium afterwards without harming the cells being cultured.

Closed Sampling For Cell Monitoring

Closed system sampling can be done in the TideXcell™ system. Each vessel types have their own corresponding number of sampling port.

A matrix cassette is packed with carriers configured in 3D for optimum cell growth. Cassettes are stacked inside the matrix vessel (2 L, 20 L, 100 L) for uniform seeding of cells.

SCADA

TideXcell™ PLC-based monitoring and control structure runs on WonderWare SCADA. This provides a high-level process supervisory management and data acquisition. This software platform will ensure process control safety, will support control strategy, and will provide a remote method of capturing data and events (alarms) for monitoring the continuous process. SCADA platforms also provide functions for graphical displays, alarms, trends and historical storage of data.

TideXcell™ is the core of Esco Aster, a contract development manufacturing organization (CDMO) that uses single-use adherent bioreactors in bioprocessing needs. Esco Aster focuses on process development, commercialization of new translational drugs (NTDs) for both humans and animals, orphan drugs, as a CMO for partner TideXcell™ factories looking to gain access in the ASEAN, ANZ, African region.

Dimension Comparison

TideXcell™ Product Specifications
TideXcell™ Incubation and Control Unit

Construction External Carcass: 304 stainless steel
Dimension 125 (W) X 70 (D) X 115 (H) cm
Weight 240 kg
Eletrical requirements AC 220V 20A, 50/60 Hz, Single phase
UPS (uninterrupted power supply) width 6kVA/4.2kW should be prepared by customers
Control Hardware (2-20L) Flexible pc-base/DAQ industrial control interface;
Solenoid valves/PID gauges/Pressure-vacuum motor
NDIR CO2 diffusion type sensor
Control Hardware (50-100 L) Siemens HMI/PLC based control structure
Solenoid valves/PID gauges/Pressure-vacuum motor
NDIR CO2 diffusion type sensor
Control Software Siemens PLC-based control and monitoring structure
Siemens HMI with 12.1" touchscreen
Development environment: TIA Protal V13 SP1
Incubator 0 - 20% CO2 PID Control
+8ºC~27ºC Operating Temperature
Front view window; LED inside lighting
Emergency power-off button
Connection CPC nickel-coated brass quick connectors
Electric connectors with locking-screw
Communication 9-pin Dsub RS-485 port : Modbus RTU protocol
2 USB Ports for import firmware / software upgrade and export trend data
Construction 264 W x 359 L X 170 H mm (10.4 x 14 x 6.7 inches)
Control Features Simple user's administration
Process page & Date viewer page
Data logging/Parameters logging/Events logging
Individual seed and amp
Cultivation conditions setting with default values
Automatic switch from seeding stage to cultivation stage up to 300 minutes
Automatic air refresing mechanism
Tidal control 800 ~ 1, 800 mL/min
Gas flow rate 0.8 – 20 LPM (depends on system size)
Alkaline addition 80 mL/min at 100 rpm
Perfusion rate 80 mL/min at 100 rpm
File export Excel (.xlx)
pH control 4~10 ± 0.1
DO control 0%~100% ± 0.5%
CO2 control 0%~10% ± 0.3%
Protection Over pressure protection (max. 1 bar)
Liquid leaking protection
Over suction protection
Over time protection

TideXcell™ Mixing System

Magnetic Stirrer Mixing System Recirculation Thermostatic Mixing System Stir-tank Mixing System
Construction Magnetic stirrer
10 L, 20 L borosilicate glass vessel
with stir bar for mixing
Recirculation Mixing with magnetic driven
bearing-less impeller
Stir mixing with pre-installed impeller
Electrical Requirements Monophase 3-threads AC 220V ± 10V
5A max 50/60 Hz
Single phase, AC 220v,
9A max, 50/60 Hz
Three phase, 230 VAC, 50/60 Hz
Capacity 10 L and 20 L 50 L, 100 L 200 L, 500 L, 1,000 L 30 L, 50 L, 100 L, 200 L, 500 L, 1,000 L
Control 100 – 1,500 rpm stir rate
RT to +45°C temperature control
Pumping rate 0 – 21 LPM
RT to +50ºC temperature control
80 – 1,000 rpm stir rate
RT to +60ºC temperature control
SIP system (sterilization in place)

The following table shows cGMP compliant customers using TideCell in their projects. Company identities and some other information are hidden due to Non-disclosure agreement (NDA) between the company and VacciXcell

SECRETED VIRUS    

Country

Cell Line

Virus Strain

Cell Density
cells/mL

Virus Titer

cGMP

SCADA/DCS

Taiwan

VERO

Japanese Encephalitis virus

4.6 x107

109pfu/ml

Yes

-

China

VERO

Rabies virus

4.0 x107

108 IgLD50/ml

Yes

-

Taiwan

VERO

Enterovirus type 71

2.6 x107

107.8 TCID50/ml

Yes

-

Taiwan

MDCK

H5N1

2.3 x107

HA=1024~2048

Yes

SCADA

Taiwan

MDCK

A(H1N1)

2.2 x107

HA=512~1024

Yes

-

Taiwan

PK

Hog Cholera

3.7 x107

106.5pfu/ml

Yes

-

China

ST

Hog Cholera

2 x107

2 mil. RID/ml

Yes

-

China

BHK-21

Rabies

4.5x107

108.5 TCID50/ml

Yes

-

Japan

MDBK

(NDA) virus for Bovine vaccine

1.29x107

109.2 TCID50/ml

Yes

 

China

Marc145

PRRSV

1.16x107

108.29 TCID50/mL

Yes

-

Spain

NDA

(NDA) Virus of Bovine Vaccine

2.6 × 107

N/A

Yes

-

NON-SECRETED VIRUS      

Country

Cell Line

Virus Strain

Cell Density

Virus Titer

cGMP

SCADA/DCS

Japan

NDA

Hepatitis A

2×1011

N/A

Yes

SCADA