Bioprocess in Action: Esco VXL™ Hybrid | Esco VacciXcell
The Esco VXL™ Hybrid is a versatile bioreactor for your research and development needs. It can do four types of cultures namely: adherent culture using macroporous carriers, adherent culture using microcarriers, suspension, and fermentation. Just switch and configure your vessel to meet your culture process! Tide Vessel for adherent and stirred vessel for suspension/fermentation.
Hybrid Bioreactor, changing perspective from discovery to delivery.
Parts:
· Hybrid Bioreactor
· Matrix Vessel is used for culturing adherent cells using the proprietary BioNOC Il macrocarriers. These macrocarriers serve as the packed bed where cells can attach to.
· Suspension Vessel is used for suspension culture with or without microcarriers and fermentation. The matrix basket is replaced by a baffle and a Pitch-blade turbine is used.
Starting the Hybrid Bioreactor
Logging in the hybrid bioreactor
· Press Log-In.
· Input username and password.
Setting the Agitation Rate
· Set desired agitation speed on the Main Page.
· Click Main Page and then Control Panel
· Click "Agit“ in the Control Panel to turn Agitation On
Setting the Temperature
· Set desired temperature on the main page.
· To turn on the temperature function, press “Temp” on the Control Panel page.
· To know which parameters are currently active, go to the main page and observe which parameters have blinking green highlights.
Tide Motion Set-Up
· To set parameters for tide motion, press “Tide Mode Setup” then “Inoculation Cultivation”.
· Change parameters as desired by pressing on the corresponding numbers.
· To start tide motion, press “Tide Auto” then “Tide Start”.
· Culture media from the inner vessel is pushed out to the outer vessel, submerging the matrix carriers in culture media. This is the nutrition phase of the tide motion process.
· After a predetermined time, the media will be pushed out of the outer vessel and back to the inner vessel, emerging the matrix carriers to air. This is the aeration phase of the tide motion process.
Process Mode
· Start the Feeding and Harvesting.
· Clamp the sampling tube.
· Attach a needleless syringe to the sampling port and create suction.
· Release the clamp to draw out media.
· Re-clamp the sampling tube before removing it to avoid contamination.
