The Esco VXL™ Hybrid is a versatile bioreactor for your research and development needs. It can do four types of cultures namely: adherent culture using macroporous carriers, adherent culture using microcarriers, suspension, and fermentation. Just switch and configure your vessel to meet your culture process! Tide Vessel for adherent and stirred vessel for suspension/fermentation.
Learn more about the product by downloading the brochure here: https://escovaccixcell.com/assets/downloadables/hybrid-bioreactor-SellSheet-A4-VB-HR.pdf
Bioprocess in Action with Adherent Tide Motion!
Hybrid Bioreactor, changing perspective from discovery to delivery.
Parts:
· Hybrid Bioreactor
· Matrix Vessel is used for culturing adherent cells using the proprietary BioNOC Il macrocarriers. These macrocarriers serve as the packed bed where cells can attach to.
· Suspension Vessel is used for suspension culture with or without microcarriers and fermentation. The matrix basket is replaced by a baffle and a Pitch-blade turbine is used.
Starting the Hybrid Bioreactor
Logging in the hybrid bioreactor
· Press Log-In.
· Input username and password.
Setting the Agitation Rate
· Set desired agitation speed on the Main Page.
· Click Main Page and then Control Panel
· Click "Agit“ in the Control Panel to turn Agitation On
Setting the Temperature
· Set desired temperature on the main page.
· To turn on the temperature function, press “Temp” on the Control Panel page.
· To know which parameters are currently active, go to the main page and observe which parameters have blinking green highlights.
Tide Motion Set-Up
· To set parameters for tide motion, press “Tide Mode Setup” then “Inoculation Cultivation”.
· Change parameters as desired by pressing on the corresponding numbers.
· To start tide motion, press “Tide Auto” then “Tide Start”.
· Culture media from the inner vessel is pushed out to the outer vessel, submerging the matrix carriers in culture media. This is the nutrition phase of the tide motion process.
· After a predetermined time, the media will be pushed out of the outer vessel and back to the inner vessel, emerging the matrix carriers to air. This is the aeration phase of the tide motion process.
Process Mode
· Start the Feeding and Harvesting.
· Clamp the sampling tube.
· Attach a needleless syringe to the sampling port and create suction.
· Release the clamp to draw out media.
· Re-clamp the sampling tube before removing it to avoid contamination.
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Esco AsterMavors Cellular Agriculture and alternative proteins (Food)
Blk71 Ayer Rajah Crescent
Singapore 139951
Esco AsterTide PD-Phase 2 CTM (Cells/Viruses/Evs)
Blk 67 Ayer Rajah Crescent
Singapore 139950
Esco Aster Fermentation (Plasmids)
21 Changi South Street 1
Singapore 486777
#RollingWithTheTide #EscoAster
Learn more here: https://escovaccixcell.com/tide_technology/cell_cultivation/TideXcell
Rolling with the Tide: TideXcell®
TideXcell™ is the gentle giant bioreactor utilizing the Tide Motion technology. Cut off laborious tasks and save costs with a bioreactor that has a high cell or product yield.
Its advanced features for cell growth and production, as well as its closed harvesting system, makes it a powerful tool in the field of bioprocessing.
We have upgraded the TideXcell® system for the bioprocessing industry. The FDA-listed gentle giant bioreactor is designed and manufactured in compliance with cGMP guidelines.
The closed, automated TideXcell® is available either with disposable components for single-use application or glass or stainless-steel components for the multiple-use application.
Central to the system’s control is the Siemens HMI/PLC monitoring system that can be connected to SCADA systems that have Delta V, Pcs7, Wonderware, or Mitsubishi controls. Optional redundant systems are also catered.
Built with quality by design features, the separation of the matrix and the mixing vessel permits a dual temperature control process and 100% media exchange for producing higher virus titers or cell yield.
Cells are seeded and attach to the BioNOC™ II or matrix cassette which allows a large surface area for adherent cell growth and expansion in a three-dimensional configuration.
These two seeding configurations make it flexible for high product yield applications such as stem cells, exosomes, lentivirus-, adeno-associated viral vector production, vaccines, recombinant proteins, and more.
The Tide Motion principle in the bioreactor boosts the scalability and productivity feature of the TideXcellᴿ system. The cells attached to the carrier are exposed to air as the culture medium goes down during the aeration phase. Alternatively, nutrients are taken up by the cells as the culture medium goes up during the nutrition phase.
The system is integrated with industrial-grade peristaltic pumps for feed, harvest, and the proprietary 100% media exchange.
100% media exchange allows 100% media harvest to feed 100% fresh culture media, even changing into a different type of media for virus growth. This is a unique advantage of Tide that makes viral titer, in some cases, higher in adherent.
Monitoring cells are quite daunting in packed-bed bioreactors, but with TideXcell®, everything is made easy. The closed system sampling port enables sampling for cell observation and needleless sampling port for glucose monitoring, without having the need to open the system up. The number of closed system sampling ports depends on the scale of the matrix vessel being used.
Real-time pH levels are measured using Mettler Toledo pH sensors.
The DO probe obtains real-time dissolved oxygen in the system.
To meet the closed automated demand of the users for harvesting cells, TideXcell® is equipped with its own TideXcell® Cell Harvesting System that follows the same conventional harvesting method but at a cell harvesting efficiency of more than 90%.
TideXcell® is a gentle Tide Motion bioreactor that breaks adherent scalability issues from pilot scale to commercial manufacturing scale. This bioreactor represents a very suitable system for adherent bioprocessing.
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Reach us through our other social media accounts!
LinkedIn: https://sg.linkedin.com/company/esco-aster
E-mail: [email protected]
Facebook: https://www.facebook.com/VacciXcell
Instagram: escolifesciences
Esco AsterMavors Cellular Agriculture and alternative proteins (Food)
Blk71 Ayer Rajah Crescent
Singapore 139951
Esco AsterTide PD-Phase 2 CTM (Cells/Viruses/Evs)
Blk 67 Ayer Rajah Crescent
Singapore 139950
Esco Aster Fermentation (Plasmids)
21 Changi South Street 1
Singapore 486777
#RollingWithTheTide #EscoAster
The TideXcell® is the Gentle Giant of Adherent bioprocessing. It is a linearly scalable bioreactor that runs on a patented Tide Motion principle intended for adherent cell culture. The Tide Motion principle provides gentle oscillation of the medium, with extremely low shear stress, foaming, and bubble-free environment for the cells. It offers linear scalability from 2L to 2000L. It also features a TideXcell® cell harvesting system for automated and closed-cell recovery. Integrated with process monitoring system features, the TideXcell® bioreactors ensure quality while generating high product yields.
Learn more about Tide Motion:
https://escovaccixcell.com/Tide_Technology
Learn more about our bioreactors: https://escovaccixcell.com/tide_technology/cell_cultivation/TideXcell
For decades the biopharma industry has been facing issues with the scale-up of adherent cell cultures, 2D cell stacks, roller bottles, and microbeads are laborious and spatially demanding.
The Tide motion platform is linearly scalable for stem cells, exosomes, lentivirus, adeno-associated virus, vaccines, and recombinant protein production.
Tide motion asides the packed bed between the nutrients phase and oxygenation phase reducing shear stress on the cells while maximizing oxygen intake. Cells are grown inside three-dimensional matrix fibers called BioNOC™ II. BioNOC™ II has been demonstrated for the expansion of VERO, MRC-5. Mesenchymal Stem Cells, HEK293T, primary cells, and more.
The TideXcell® consists of an incubator which is fitted with a VOC and dual-HEPA filters connected to a mixing vessel or any single-use bag. Inside the TideXcell™ incubator is the matrix vessel which can be monitored by the touchscreen Siemens HMI PLC. The TideXcell® supports SCADA control systems.
In-process monitoring is an integral part of quality assurance BioNOC™ II macrocarriers can be easily sampled to obtain a snapshot of cell culture processes. The mixing vessel controls the temperature of the culture medium. The distinct separation of the mixing vessel and the matrix vessel allows for a dual temperature control model that helps to produce higher yields.
From the lab scale to the pilot scale to the industrial scale. The tide motion platform features a range of different bioreactor capabilities. Both single-use and multiple-use technologies are available to fit your specific application and budget. The tied motion platform covers the sea train with a modular choice of bioreactors that fit your process development. The tide motion platform integrates with the Esco cell processing isolator as a highly modular system for cell, gene therapy, tissue engineering, and cell processing.
The TideXcell® harvester is a flexible and versatile unit suitable for seed cell banking.
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Reach us through our other social media accounts!
Website: http://www.escoaster.com/
LinkedIn: https://sg.linkedin.com/company/esco-...
E-mail: [email protected]
Facebook: https://www.facebook.com/VacciXcell
Instagram: escoglobal
Esco Aster Pte Ltd (CDMO Services)
#02-09
Block 67
Ayer Rajah Crescent
Singapore 139950
Esco Aster BTI Joint Lab Allogeneic Car T
Centre of Excellence in Bioprocessing
# 05-01, Centros 20 Biopolis Way, Singapore 138668
+65 6256 7950
Esco Gb Ltd
Unit 2 R-Evolution @ Gateway 36, Kestrel Way, Barnsley, S70 5SZ
E-mail: [email protected]
Tel: +44 (0) 01226 360799
#RollingWithTheTide #TideMotion
A complete time-lapse of cell expansion using CelCradle™ as a seed train for scaling up to the TideXcell® system. Cells are harvested through the automated TideXcell™ Harvesting System, achieving cell recovery of more than 90%.
Learn more about the Tide Motion platform:
https://escovaccixcell.com/Tide_Technology
Rolling with the Tide:
CelCradle™ to TideXcell® to Cell Harvesting System (Timelapsed R&D Bioprocess)
- Harvesting from CelCradle™
- Preparation of sterile mixing vessel
- Preparation of media
- Setting up Tide Motion parameters using SCADA
- Setting up monitoring and control parameters
- Inoculation of cell culture
- Cell cultivation on BioNOC™ II macrocarriers
- Monitoring of media glucose
- Sampling and monitoring of cells
- Sampling of carriers using closed sampling port
- Harvesting of cells using TideXcell® Cell Harvesting System
Note: Please refer to the manuals and protocols provided; this time-lapsed video is for demonstration only.
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Reach us through our other social media accounts!
LinkedIn: https://sg.linkedin.com/company/esco-aster
E-mail: [email protected]
Facebook: https://www.facebook.com/VacciXcell
Instagram: escolifesciences
Esco AsterMavors Cellular Agriculture and alternative proteins (Food)
Blk71 Ayer Rajah Crescent
Singapore 139951
Esco AsterTide PD-Phase 2 CTM (Cells/Viruses/Evs)
Blk 67 Ayer Rajah Crescent
Singapore 139950
Esco Aster Fermentation (Plasmids)
21 Changi South Street 1
Singapore 486777
#OneWorldOneHealth #EscoAster #Bioreactor
Learn more about the CelCradle™ here: https://escovaccixcell.com/tide_technology/cell_cultivation/celcradle
The CelCradle™ operates through the Tide Motion principle wherein cells, attached to BioNOC™ II carriers, are alternately exposed to aeration and nutrition via the decompression and compression of the bellows holding the culture medium. The gentle vertical oscillation of the culture medium creates a dynamic interface between air and culture medium on the surface of the cells, providing the cells with an environment that is of low shear stress, high aeration and nutrition levels, zero foaming, and no O₂ limitation. This efficient nutrient and oxygen transfer allows the CelCradle™ system to produce high cell density yield.
The following video will show how to seed adherent cells in the CelCradle™ system.
In the Biosafety cabinet, prepare seed in 120ml of fresh and pre-warmed culture medium.
- Ensure pH is between 7.2-7.4.
- Dispense seed mixture on the carriers.
- Place the blue vented cap in a sterile petri dish and cover the dish to ensure the sterility of the cap for future use.
- Cap bottle with non-vented white cap.
- Invert bottle.
- Ensure that all the 120 mL seed mixture drains down from the bellows to the cap. Make sure that the carriers are submerged in the seed mixture.
- Swirl well to ensure cells are spread evenly.
- Move the bottle into the CO₂ incubator.
- Allow cells to attach for up to 4 h.
- Swirl the bottle gently every 15 min to allow cells to be redistributed.
- At the end of the incubation period, remove the bottle and sample 10 ml seed mixture to check the number of cells unattached.
- Alternatively, check for the number of cells attached by harvesting cells from 3 carriers.
- Top up media in a bottle to 500 ml.
- Cap the bottle with the vented blue cap. The non-vented white cap should be left in the sterile petri dish and covered to ensure its sterility for further use.
- Move the bottle into the CelCradle™ Stage. Set up Tide Motion parameters and press “Start”.
- Parameters can be adjusted according to cell line characteristics and media conditions.
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Reach us through our other social media accounts!
Website: http://www.escoaster.com/
LinkedIn: https://sg.linkedin.com/company/esco-...
E-mail: [email protected]
Facebook: https://www.facebook.com/VacciXcell
Instagram: escoglobal
Esco Aster Pte Ltd (CDMO Services)
#02-09, Block 67
Ayer Rajah Crescent
Singapore 139950
Esco Aster BTI Joint Lab Allogeneic Car T
Centre of Excellence in Bioprocessing
# 05-01, Centros 20 Biopolis Way, Singapore 138668
+65 6256 7950
Esco Gb Ltd
Unit 2 R-Evolution @ Gateway 36, Kestrel Way, Barnsley, S70 5SZ
E-mail: [email protected]
Tel: +44 (0) 01226 360799
#MakingScienceMoveToYourAdvantage #EscoAster
Learn more about the CelCradle™ here: https://escovaccixcell.com/tide_technology/cell_cultivation/celcradle
The CelCradle™ operates through the Tide Motion principle wherein cells, attached to BioNOC™ II macrocarriers, are alternately exposed to aeration and nutrition via the decompression and compression of the bellows holding the culture medium. The gentle vertical oscillation of the culture medium creates a dynamic interface between air and culture medium on the surface of the cells, providing the cells with an environment that is of low shear stress, high aeration and nutrition levels, zero foaming, and no O₂ limitation. This efficient nutrient and oxygen transfer allows the CelCradle™ system to produce high cell density yield.
A proper cell harvesting method is important to get the maximum cell yield at the end of the culture process.
The succeeding video will show how to harvest cell properly from the CelCradle™ system
- To start off, remove the CelCradle™ bottle from the incubator.
- Under a hood, uncap the bottle and place the blue vented cap into the petri dish to ensure sterility.
- The strainer is autoclavable. Keep n mind after the whole process of cell harvesting, the strainer should be autoclaved.
- Using the strainer, drain off the spent culture medium into the waste beaker.
- Tap the strainer gently to allow any remaining carriers to fall back into the bottle.
- Gently wash the cells with 500 ml of PBS or phosphate-buffered saline, repeating the process twice, and place the bottle back into the CelCradle™ stage and set the parameters.
- The speed for the up & down rate should be set at 2.0mm/s and the top and down holding time should be set at 0.
- Gently adding the PBS into the bottle Gently rinsing of PBS ensures that only the serum & non-viable cells are washed off.. This step is to increase cell viability during harvesting.
- After 5 minutes of incubation, remove the bottle from the CelCradle™ stage.
- Drain the PBS solution using a strainer. Subsequently, repeat the rinsing steps using PBS.
- Add 120ml of pre-warmed dissociation solution and capped the bottle with a non-vented white cap.
- Then, invert the bottle & swirl it to ensure all carriers are submerged in the solution.
- Place the inverted bottle into the incubator.
Note: If cells do not come out easily, invert the bottle and place the bottle into an orbital shaker set at 37 degrees celsius for 15 minutes at 200 rpm. This is to ensure complete cell harvest. Sufficient time for enzymatic digestion is critical for successful cell harvesting.
- Once the incubation period is done, retrieve your bottle from the incubator and head to the biosafety hood.
- Uncap your bottle & pour out the dissociation solution into the water beaker using the Cel Cradle strainer.
- Add 100ml of a solution containing 0.5mg/ml of DNAse & 0.1g/ml trypsin inhibitor solution into the bottle & firmly cap the bottle with a non-vented white cap.
- Invert & swirl the bottle to wash off cells from the carriers.
- Tap the bottle sharply & steadily against your palm. Rotate the bottle & tap each succeeding corner 15 times vigorously.
Note: Using trypsin inhibitor is encouraged as the serum will cause foaming, preventing cells from being dislodged during harvesting. It also aids in detaching cells from the carriers.
- Swirl the carriers to detach the cells (tapping and swirling should be done twice).
- Transfer the solution from the beaker to the centrifuge tube to spin down the cells.
- Harvest all cells through centrifugation after each round of cell harvest.
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Reach us through our other social media accounts!
Website: http://www.escoaster.com/
LinkedIn: https://sg.linkedin.com/company/esco-...
E-mail: [email protected]
Facebook: https://www.facebook.com/VacciXcell
Instagram: escoglobal
Esco Aster Pte Ltd (CDMO Services)
#02-09, Block 67
Ayer Rajah Crescent
Singapore 139950
Esco Aster BTI Joint Lab Allogeneic Car T
Centre of Excellence in Bioprocessing
# 05-01, Centros 20 Biopolis Way, Singapore 138668
+65 6256 7950
Esco Gb Ltd
Unit 2 R-Evolution @ Gateway 36, Kestrel Way, Barnsley, S70 5SZ
E-mail: [email protected]
Tel: +44 (0) 01226 360799
#MakingScienceMoveToYourAdvantage #EscoAster
Learn more about our Tide Motion Bioreactors: https://escovaccixcell.com/tide_technology/cell_seeding/tide_motion_bioreactors
An adequate number of cells is required for inoculation in a particular bioreactor. Starting from cells thawed from the bank, cells are then cultivated through a series of cultivating systems and passages. Each passage becomes larger and would require higher volumes of media and other resources. At times, further optimization is needed to replicate the fold increase with each seed train.
Esco VacciXcell bioreactors are linearly scalable for each seed train, reducing the time required for the optimization of the process for generating seed trains.
BioNOC™ II is a macroporous carrier that supports the growth of anchorage-dependent cells including animal, mammalian, and insect cells in either serum-containing or serum-free culture media. It is made of 100% pure polyester nonwoven fabric manufactured according to cGMP guidelines. The special geometric design and surface treatment on the carrier enhance fluid mixing, immobilization efficiency, protection from shear forces, and nutrient transfer during cell culture.
The following video will show how to test BioNOC™ II macrocarriers for MiniTideᴿ run:
Cell Seed Preparation:
- Place your autoclaved carriers inside the biosafety cabinet.
- Aspirate phosphate-buffered saline (PBS) and aseptically transfer autoclaved carriers into a 50mL tube.
- Inoculate carriers by pipetting the required number of cells into the 50mL tube.
- Tilt and rotate the tube to make sure all carriers are submerged with the seed cells.
Note: Do not rotate beyond 90° to minimize the risk of cell loss.
- Loosen the cap of the tube and incubate for 3 to 5 hours.
- At the first hour of incubation, tilt and rotate the tube every 15 minutes.
- For the succeeding hours, repeat the process at a 30-minute interval.
- After 3 or more hours of incubation, sample 50uL media and perform cell count. If an attachment rate of 90% is achieved, proceed to cell culture.
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Reach us through our other social media accounts!
LinkedIn: https://sg.linkedin.com/company/esco-aster
E-mail: [email protected]
Facebook: https://www.facebook.com/VacciXcell
Instagram: escolifesciences
Esco AsterMavors Cellular Agriculture and alternative proteins (Food)
Blk71 Ayer Rajah Crescent
Singapore 139951
Esco AsterTide PD-Phase 2 CTM (Cells/Viruses/Evs)
Blk 67 Ayer Rajah Crescent
Singapore 139950
Esco Aster Fermentation (Plasmids)
21 Changi South Street 1
Singapore 486777
#MakingScienceMoveToYourAdvantage #EscoAster #MiniTide #Bioreactors #LinearlyScalable #BioNOC #CellSeeding
