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TideXcell® Harvesting System

The Gentle Giant's Harvester Partner
The TideXcell® Harvesting System (TXLHS) is specially designed for TideXcell™ high-density cell culture system for cell recovery from the matrix vessel (2 L, 20 L, 100 L volume). The TXLHS is a powerful automatic cell harvester to harvest and transfer viable cells from large-scale TideXcell® system in a closed and automated system; eliminating the risk of contamination. It can also be applied to harvest cell mass for biological production and any related applications. With the use of TXLHS, up to 1011 cells can be harvested within one (1) to three (3) hours operation of the cell harvester.
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TideXcell<sup>®</sup> Harvesting System

Tide Motion in TideXcell® Harvesting System


Features

  • Whole harvesting run can be setup from washing to cell harvesting in a closed, automated manner.
  • Suitable for cell harvest from TideXcell™ 2L matrix vessels to 20L matrix vessels.
  • Support the cell seed source for TideXcell™-010 up to TideXcell™-100 systems.
  • Harvest viable animal cells within 1 to 3 hours (harvest time depends on harvest cycles).
  • Up to 1011 cells with viability above 95% could be recovered from one THS unit
Note: Cell harvest efficiency depends on the cell line used.

Applications

  • Automated cell harvesting of adherent cell culture including but not limited to: Insect Cells, Mammalian Cells, Stem Cells
  • Biomass expansion for seed train from 2D to 3D
  • Autologous and allogeneic cell therapy
  • Intracellular viruses

Highlights

  1. Installation
    Disconnect the TideXcell® matrix vessel with the mixer. Mount and fix the TideXcell® matrix vessel in the THS and connect the vessel with tubing manifolds that provide the sources. The six sources connect with the matrix vessel through 6 peristaltic pumps.
  2. Rinse
    Rinse the matrixes with PBS or equivalent solution to remove serum that could inhibit the enzymatic activity in the following step.
  3. Enzymatic Digestion
    Pump in and out chosen dissociation enzyme or equivalent solution to digest cells from the matrix. Incubate matrix vessel after pumping of enzyme.
    *Incubation time might be longer or shorter depends on the condition and needs to be determined beforehand
    Note: Incubation time might be longer or shorter depending on the cell culture requirements. This needs to be determined beforehand prior to running the cell culture harvester.
  4. Inhibitor
    Gently pump in and out enzyme inhibitor to stop the enzymatic reaction.
  5. Detach
    Automatically shake the matrix vessel. Parameters such as time and force for detachment are inputted in the system.
  6. Harvesting
    Pump in culture media, shaking and rolling the matrix vessel to let cells fall off from the matrix.
    Pump out the media to harvest tank, this media includes suspended cells.
  7. Repeat No.5 - 6
    Repeat 5 times on average

Parameters

  • Pump speed
  • Rotation speed
  • Duration of shaking/rotating
  • Harvesting cycles
Frequently Asked Questions
Downstream processing involving harvesting should take into consideration the type and purity of the target product. Harvest of secreted products depends on whether microcarriers are used. Non-secreted products require cell lysis to release the intracellular targets. Live cells also depend on whether the culture is adherent or suspension.
The system uses a combination of enzymatic (through the addition of digestive enzymes) and mechanical (shaking and rotating) to efficiently harvest the cells.
The harvesting duration depends on the inputted harvest cycles. Run usually takes 1 to 3 hours.
Events and alarms data can be exported to CF/SD card.
Yes, it is recommended to determine the flow rate of the pumps to ensure the correct volume of reagents added and waste removed from the matrix vessel.
Adherent cell culture cells can be harvested including insect, mammalian, and stem cells
Intracellular viruses such as CEF and Maerek can be harvested to release the virus while maintaining particle integrity.
The TideXcell® can accommodate TideXcell® 2L and 20L matrix vessels.

Product Specification

TideXcell® Harvesting System (TXLHS) Technical Specifications

Infrastructure Exterior dimesion (WxDxH) Controller unit:
80cm x 91cm x 181.2cm
Main chamber:
110cm x 110cm x 188.7cm
Weight 1,330 kg not including 2/20L adaptor (18kg)
Power Single phase, AC, 220 V, 50/50 Hz
Gas Input At least 0.5 MPa from the air source
Other RS485 communication port
Function: Rotation Functional unit Rotary motor
Work 750 W
Range 0°~180° (back and forth)
Rate 0~10 rpm ± 2 rpm
Period 0~999 sec ± 5 sec
Function: Shake Functional unit Shake motor
Work 3.5 kW at 300 rpm
Range 13cmmax
Rate 50~300 rpm ± 10 rpm
Period 0~999 sec ± 5 sec(16.6 mins)
Function: Break Functional unit Damper
Function: Feed and Harvest Functional unit 6 Peristaltic pumps (Harvest Out, Buffer Saline In, Enzyme In, Inhibition In, Culture Medium In, Waste Recovery)
Rate 20 - 250 rpm ± 5 rpm
Period 0~999 sec ± 5 sec
Tubing ID 9.6 mm(3/8")*WT 32 mm(1/8")
Consumable: Matrix vessel Volume 2 L and 20 L
Material PP
Consumable: Tubing set Type ID:3/8", OD:5/8"
Tool: Torque wrench Range 10~45 N•m
Control: Touch panel Size 10.4" color
Modes Automated and manual
Efficiency Cell recovery rate 2L Vessel: >90% recovery rate
20L Vessel: 70%~90% recovery rate