Virus Production Questionnaire

Please answer the following questions as completely as possible. The information here will be kept with utmost confidentiality and will only be used to generate a customized protocol for your facility.

I. Customer Information

Contact Person

Company Name

Designation

Contact Number

Department

Email Address

II. Experimental Details

1.Target Product

Secreted Virus

Non-secreted Virus

Others

2. Cell Type

Adherent cell

Suspension cell

3. What is the intended use for the product? e.g. animal vaccine, clinical phase, raw material for clinical trials

4. What is the analytical technique for measuring viral titer?

5. Target viral titer, volume and yield

Titer (pfu/mL):

Volume (L):

Yield (pfu):

6. Current titer, volume and yield

Titer (pfu/mL):

Volume (L):

Yield (pfu):

5. Any animal serum at any point in the process?

Yes, what percentage?

6. Is the media a chemically defined formula?

Yes, chemically defined

No, contains animal derived products Media description:

7. What is the cell density?

Seeding Cell Density:

Cell Density at first harvest:

Cell Density at last harvest:

8. Virus name and strain

9. Please describe the virus strain morphology, e.g. ds/ss DNA, ds/ss, +/- RNA, any lipid envelope, temperature sensitivity, surface proteins, etc： ds / ss DNA，ds / ss，+/- RNA

16. Is there CPE (Cytopathic effect) after infection? When?

Yes;

hours post infection

Describe the CPE:

Not Sure