Protein Production Questionnaire

Please answer the following questions as completely as possible. The information here will be kept with utmost confidentiality and will only be used to generate a customized protocol for your facility.

I. Customer Information

Contact Person

Company Name

Designation

Contact Number

Department

Email Address

I. General Details

1. Target Product

Secreted Protein

Monoclonal Antibody

Non-secreted Protein

Others

2. Cell Type

Adherent cell

Microbial

Suspension cell

Stem Cell

II. Experiment Details

a. Cell Culture

1. Cell Line

CHO

Hybridoma for IVD (In vitro Diagnostics)

HEK 293

Hybridoma for Therapeutics

Others please Specify

2. Any special features or peculiarities of the cell line or methods

3. Intended Use

Human Use (Production)

Human Use (Research)

Animal Use (Production)

Animal Use (Research)

17. Current System Annual dose (product quantity)

18. Current System average total cell density (per single system eg., per 1 roller bottle)

Seeding Cell Density:

Harvesting End Cell Density:

19. Do you have scale up plan?

Yes

20. Expected Scale when scaled up (Cell Density, Doses etc.)

b. Protein Production

21. Protein extraction method

Cell Harvest

Freeze/Thaw

Medium Harvest

Lysis Buffer

Others:

22. Harvesting process for medium harvest extraction method

Single harvest

Multiple harvest;

Interval time hours for days

Others:

c. CelCradle^® System

23.

Seeding 1 – 3 x 10⁸ cells be difficult?

Yes

If yes, how many cells do you plan to seed?:

24.

Will the CO₂ incubator be exclusively used for the CelCradle^® System?

Yes

25.

Can you adjust the CO₂ concentration of incubator?

Yes

26. What are the challenges / limitations you experience with your current system?

27. What is your expectation using our system?

28.

Is there any changes required from your existing process protocol?

Yes

29.

With Tide-motion bioreactor, is it okay to change the process protocol?

Yes

Protein Production Questionnaire

a. Cell Culture

b. Protein Production

c. CelCradle® System

c. CelCradle^® System